Each chapter in this book corresponds to a lab in the CHEM 3753: Introduction to Biochemical Methods course at the University of Oklahoma. All of the materials you will need for each lab can be found within its respective chapter. Each chapter will contain a brief introduction; a set of learning objectives; a slide presentation, screencast, or lab demonstration video; the protocol to be followed during your time in the lab; and a set of interactive quiz questions to help you check you understanding as you go. Other interactive features include photos from the lab, links to safety data sheets (SDS), and 3D chemical structures. Components of the book are elaborated upon below. We hope you find this book to be an all-in-one, fun, and engaging learning tool for the biochemical methods course.
Each chapter has a brief introduction to help orient you with the material to be covered in the coming lab. This introduction is found in itallics on the title page for each chapter.
After opening a chapter, you will see a list of Learning Objectives in a column to the left of your screen. These objectives are there to help you focus on what you will need to learn in each chapter. Consider these your road map to navigating the chapter material.
Each chapter in this book contains a review in the form of a slide presentation or screencast video. These reviews are a lot like lectures, and they are intended to help you brush up on the theory that will be applied in the lab. Reviews appear at the beginning of each chapter. Start by clicking through the slides or viewing the screencasts to get a better grasp on the concepts you will encounter in the lab.
Lab Demonstration Videos
Some chapters contain videos that demonstrate specific techniques to be performed in the lab. These include techniques like pipetting and pouring an agarose gel, which involve a series of steps that need to be performed repeatedly and with accuracy. We have filmed the videos in the biochemical methods teaching labs at the University of Oklahoma such that what you see in the video is what you will find in the lab – no surprises! We encourage you to watch the relevant video(s) prior to coming to the lab and to return to them whenever you need a refresher.
Each chapter contains its corresponding laboratory protocol, including a list of the materials you will need to perform the experiment at hand. Read the protocol carefully before coming to the lab to understand what you will be doing and how you will be doing it. Bring this digital book or its printed version to the lab to review the protocol, tables, and figures while you work.
Near the end of each chapter, you will find interactive quizzes that ask you to apply what you have learned in lecture and in the lab. These quizzes are there to help you gauge you understanding of the material that has been covered. Simply read the questions and select an answer, and you will be told whether your answer is correct or incorrect. Note that there are no points attached to these quizzes and that no one will be told how you perform on them. They are there for you own benefit, so take them as many times as you need.
Other Fun Things
We have included in this digital book, pictures from the biochemical methods teaching labs, 2 and 3-D chemical structures, interactive structures, interactive graphics, as well as hyperlinks to safety data sheets (SDS) and supplementary materials that may be of use to you as you learn. Please make use of these features and let us know your ideas about how we can make this book even more beneficial to future students.